Journal: RSC Advances

Article Title: Novel deep eutectic solvent-based liquid phase microextraction for the extraction of estrogenic compounds from environmental samples

doi: 10.1039/d2ra01754g

Figure Lengend Snippet: The effect of the molar ratio of HBA to HBD (A), volume of DES (B), KOH concentration (C), volume of HCl (D), time of derivatization (E) and derivatization before and after extraction (F) on the extraction efficiency of estrogenic compounds obtained from DES-LPME/GC-MS.

Article Snippet: Estrogenic compounds (E1, E2, E3 and EE2 with purity higher than 97%), strone 3-methyl ether (internal standard, I.S.), N , O -bis(trimethylsilyl) trifluoroacetamide (BSTFA) with trimethylchlorosilane (BSTFA + TMSC, 1%), l -menthol and (1 S )-(+)-camphor-10-sulfonic acid (CSA) were obtained from Sigma-Aldrich Chemie (St. Louis, Missouri, USA).

Techniques: Concentration Assay, Extraction, Gas Chromatography-Mass Spectrometry

Journal: RSC Advances

Article Title: Novel deep eutectic solvent-based liquid phase microextraction for the extraction of estrogenic compounds from environmental samples

doi: 10.1039/d2ra01754g

Figure Lengend Snippet: Analytical characteristics of DES-LPME/GC-MS for determination of estrogenic compounds in environmental water and wastewater samples

Article Snippet: Estrogenic compounds (E1, E2, E3 and EE2 with purity higher than 97%), strone 3-methyl ether (internal standard, I.S.), N , O -bis(trimethylsilyl) trifluoroacetamide (BSTFA) with trimethylchlorosilane (BSTFA + TMSC, 1%), l -menthol and (1 S )-(+)-camphor-10-sulfonic acid (CSA) were obtained from Sigma-Aldrich Chemie (St. Louis, Missouri, USA).

Techniques:

Journal: RSC Advances

Article Title: Novel deep eutectic solvent-based liquid phase microextraction for the extraction of estrogenic compounds from environmental samples

doi: 10.1039/d2ra01754g

Figure Lengend Snippet: Relative recoveries and standard deviations of estrogenic compounds from spiked water and wastewater samples

Article Snippet: Estrogenic compounds (E1, E2, E3 and EE2 with purity higher than 97%), strone 3-methyl ether (internal standard, I.S.), N , O -bis(trimethylsilyl) trifluoroacetamide (BSTFA) with trimethylchlorosilane (BSTFA + TMSC, 1%), l -menthol and (1 S )-(+)-camphor-10-sulfonic acid (CSA) were obtained from Sigma-Aldrich Chemie (St. Louis, Missouri, USA).

Techniques:

Journal: RSC Advances

Article Title: Novel deep eutectic solvent-based liquid phase microextraction for the extraction of estrogenic compounds from environmental samples

doi: 10.1039/d2ra01754g

Figure Lengend Snippet: Comparison of the present method with other methods applied for the determination of estrogenic compounds

Article Snippet: Estrogenic compounds (E1, E2, E3 and EE2 with purity higher than 97%), strone 3-methyl ether (internal standard, I.S.), N , O -bis(trimethylsilyl) trifluoroacetamide (BSTFA) with trimethylchlorosilane (BSTFA + TMSC, 1%), l -menthol and (1 S )-(+)-camphor-10-sulfonic acid (CSA) were obtained from Sigma-Aldrich Chemie (St. Louis, Missouri, USA).

Techniques: Comparison

Journal: Redox Biology

Article Title: Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females

doi: 10.1016/j.redox.2023.102986

Figure Lengend Snippet: CYP1B1-related metabolites and effect of estrogen treatment on mitochondria of human corneal endothelial cells and CYP1B1 protein expression in normal and FECD cell lines. (a) Illustration of the human eye showing normal compared to FECD corneal endothelium. (b) Effect of NQO1 deletion on CYP1B1 expression, and the role of CYP1B1 specific inhibitor, TMS and NRF2-ARE-NQO1 agonist, berberine in estrogen metabolism. UPLC/MS/MS analysis of CYP1B1-related metabolites showing upregulation of (c) harmful catechol estrogens (4-OHE 1/2 ) in aqueous humor (n = 6,*p < 0.05; normal and FECD), (d) increased estrogen DNA adducts (4-OHE 1/2 -DNA), (e) downregulation of non-harmful 4-OHE 1/2 catechol conjugates in ex vivo corneal endothelial tissue of FECD (pooled n = 12,*p < 0.05) compared to normal samples (non-pooled n = 8,*p < 0.05). (f) Expression of CYP1B1 protein in normal male (SVN3-65M), FECD male (SVF3-76M), normal female (SVN1-67F), and FECD female (SVF1-73F) cell lines in response to 2,3,7,8 -Tetrachlorodibenzo-p-dioxin (TCDD, 30 nM). (g) Bar graph showing significantly increased CYP1B1 expression in SVF3-76M, SVN1-67F and SVF1-73F post TCDD (n = 3,*p < 0.05,**p < 0.01). (h) FECD ex vivo tissue (n = 4) showing exogenous estrogen (E2-biotin, red) colocalized (yellow) to mitochondria (MitoTracker, green) represented by Pearson's correlation; stained for nucleus (DAPI, blue).

Article Snippet: The cells were refreshed with modified Chen's media containing 0.3 μM of biotin-labeled estrogen (E2-biotin) complex (#MBS537732, My BioSource, USA) followed by treatment with or without 5 J/cm 2 UVA and recovered after 6 h of incubation in the same media.

Techniques: Expressing, Tandem Mass Spectroscopy, Ex Vivo, Staining

Journal: Redox Biology

Article Title: Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females

doi: 10.1016/j.redox.2023.102986

Figure Lengend Snippet: Estrogen induces mtDNA damage and CYP1B1 translocates to the mitochondria post-UVA in the Nqo1 −/− cell line. (a) Aldehyde Reactive Probe (ARP, green), co-localized (yellow) with TOM20 (red) in NQO1 +/+ and NQO1 −/− cells after 6 h of exposure to exogenous estrogen (E2-biotin) alone, UVA alone, or a combination of E2-biotin and UVA. ARP was also detected in the nuclei (DAPI, blue). Co-localization (yellow) of apurinic sites (ARP, green) with mitochondria (TOM20, red) analyzed by R-value and mitochondrial morphology analyzed by mitochondrial fragmentation count (MFC) respectively after (b, f) No treatment (c, g) E2-biotin (d, h) UVA and (e, i) UVA + E2-biotin treatments in NQO1 +/+ and NQO1 −/− cells (n = 30 cells counted per condition, *p < 0.05). (j) Mitochondrial and (k) nuclear DNA damage quantified by LA-qPCR in NQO1 +/+ and NQO1 −/− cells 24 h post-UVA (5 J/cm 2 ) (n = 3, *p < 0.05). (l) Western blot and quantification of CYP1B1 in the mitochondrial fractions of NQO1 +/+ and NQO1 −/− cells at 24 h post-UVA (n = 3, *p < 0.05). (m) Representative Western blot image and quantification of cytochrome c release in the whole cell lysate at 24 h post E2-biotin (n = 3, *p < 0.05).

Article Snippet: The cells were refreshed with modified Chen's media containing 0.3 μM of biotin-labeled estrogen (E2-biotin) complex (#MBS537732, My BioSource, USA) followed by treatment with or without 5 J/cm 2 UVA and recovered after 6 h of incubation in the same media.

Techniques: Western Blot